BIOLOGY 3096 (W204) - CELL STRUCTURE, FALL, 2011 (in process - preliminary version)

Instructor: Dr. Sheffield: Office, Bio-Life 311, Phone: 204 8839, e-mail jbs@temple.edu

Laboratory Coordinator::Jennifer Emtage: Office, Bio-Life 248a, Phone 204 8870, e-mail jemtage@temple.edu

Texts:

(1) Karp, G. Cell and Molecular Biology, 6th edition. J. Wiley & Sons, publisher. This is a new edition, but it is not that different from the 5th edition.  I believe that either one will be fine for this course.  The book will be available in the Temple Bookstore, of course, but you can also get it online. However, you should be aware that I have worked out a couple of interesting options with the publisher, and I don't know if they will be available in other venues. I haven't seen the bookstore prices yet, so you might want to check.

B. The Loose-Leaf. This includes the full text, but unbound, and punched for a 3-hole loose-leaf binder (not provided). I have been told that this version should result in a roughly $50 saving over option A. It would include Take Note.

C. Online:  “Course Smart” allows you to “subscribe” –that is, you have access to the text, fully searchable, for 180 days.  They also have a download option, in which you can put the book on your own hard drive.  Somehow, there is a timer in this version as well, so it becomes inaccessible after the time is up.

D.  You can also use the used book market, or the international version (if you can get it).  Prices and amount of yellow highlighter will vary.


Here is an attempt at a summary:

Karp, Cell and Molecular Biology, 6th edition

 

Bound version with Wiley Plus

Bookstore or phone order

ISBN

9780470483374

Price

164.95 list

Loose-leaf version

Bookstore or phone order

978-0-470-89465-1

114.95 list

Course Smart , http://www.coursesmart.com/9780470042175

5th edition (either online or download)

Web Site

9780470042175

80.50 

http://www.coursesmart.com/9780470483374 (online only) 6th edition

Web Site

9780470564851

85.50 

Desktop Downloadable version

Store.vitalsource.com

Web Site

 

9780470563892

102.50

Jones/Bartlet lab notebook.

 

Bookstore

 

14.00

Lab Manual

Conwell/Ritter copy center

 

About 16.00

 

Students in a hurry can buy directly from Wiley, by calling Customer service 800 225-5945, and purchase with a credit card. They just need to be sure to give them the correct ISBN to get the version they want with the proper components, hard copy text, loose-leaf version of just WileyPlus access card version. If you buy directly from Wiley the price would be similar to what the bookstore charges. You can not do this through the Wiley web site. You must use the old fashioned technology of the telephone.

 (2) You will also be expected to purchase a lab manual packet from the Copy Center, on the sixth floor of Conwell Hall or at Ritter. The lab manual includes a CD which has copies of some of the software that you will use in lab.

(3) you will need the laboratory safety manual, if you don't already have it,.

(4) A Lab notebook with carbons and removable pages.


Course Information:

This is a basic introductory course in modern cellular biology. You should have taken Biology 1111 and 2112, and Inorganic and Organic Chemistry. and should have had or be taking Physics. If you do not meet these prerequisites, you should withdraw from the course NOW.

My goals in this course are to present the evidence for our present understanding of Cell Structure, and to discuss the nature of research as well as the results. For this reason, I devote a substantial portion of the course to analysis of techniques of Cell Biology and to the history of discovery. Naturally, this can't be done for every subject, but I try to establish an environment in which it is expected that any student might interrupt and ask me to justify any statement by citing an experiment. Similarly, this is one of the approaches that I will use in exams.

I treat the text as a resource. I expect you to be able to use it to fill in areas I might have glossed over, and to provide an explanation that might be complementary to any that I provide. There are no formal reading assignments.  I have listed readings as a guide to the material. You may find additional information elsewhere in the text.

Grading will be based primarily on a class curve. About 10% of the class will receive A, 20% B, 50% C, and the rest something else. There will be two exams during the semester, each worth 20% of the final grade. The final exam will be informally divided into two parts; the first will cover the last third of the semester, and the second will be a cumulative review. The final is worth 40% of the grade. The laboratory is a required part of the course, you will receive a number grade which will count for 20% of the course grade. Thus, the maximum number of points you can accumulate will be 500. In previous interations of the course, students who have received a total of about 450 points have received A's, 400 points have received B's, and 300 points have received C's. More details of the grading procedure will be discussed in class. There will be no makeup exams. If you are forced to miss one of the mid semester exams, your grade will be based on the others, as long as I receive appropriate explanations (Doctor's note, etc.). In the absence of an excuse, the exam will count as a zero. If you miss both of the mid semester exams, you should drop the course.

Because of the number of students, exams will be multiple choice. After each exam the correct answers will be posted outside the lab. For the mid-semester exams, students who feel that their answer to a particular question is correct even though it is not the same as the posted answer may write up a detailed justification for their answer and give it to me. Students will have two class days after the exam is returned to hand in written justification for changes in the grading. Because of time constraints, I will not be able to review final exams in this way.

Since we all hate multiple choice exams, I have come up with a way to try to beat the system.  Here’s the deal.  Each week, you may send me a single multiple choice question based on that week’s material. 

Provide:

1.. the question,

2.  five possible answers, and

3.  your choice for the correct answer

4.  the rationale for your answer.  (you will not receive credit if this is omitted)

You will then receive 1 point towards your total grade for each question. You can submit only one question per week, and it must be based on that week’s material.  You may not accumulate questions and then submit them all at once.  Questions should be addressed to a special e-mail address, jbs.questions@gmail.com, and must have “Multiple Choice” in the subject line.  Since I will be collecting these automatically into a folder, they must have those words in the subject line.  I will ignore any others.  My purpose is severalfold.  First, this is a subterfuge to force you to think about the material as it goes along.  Secondly, it gives me some idea of how well you are following the course.  Thirdly, it gives you the experience of designing a multiple choice question (It’s not that easy!).  Fourthly, it saves me a little work in making up questions, since I will use some of the questions in my exams (generally with some editing).  Fifthly, if your question appears on the exam, you will already know the answer!  The maximum number you can receive is 10 points.

Office Hours: I will have office hours from 10-11:30 on Mondays, and 3:30-5 on Wednesdays and Thursdays. I will try to be available after most classes. . If you miss me then, please call for an appointment (204 8839), or take a chance and drop in. You can also send me a note by e-mail at jbs@temple.edu .I strongly recommend that you come at any time to discuss aspects of the course which are either difficult or exciting. My office is room 311 in the Biology- Life Sciences Building

We will be using Blackboard for posting notes, lab information and general class communication via e-mail. I hope that this will be an opportunity for you to share questions and answers with me and the laboratory staff. There will be more on this as the course progresses. I have posted a copy of this syllabus on the Blackboard site and on the following website: http://astro.temple.edu/~jbs/courses/204intro.html . Within the syllabus are links to the lecture notes and to some supplementary material. The lecture notes are essentially copies of the images that I present during lecture. It will be up to you to fill in with the text and jokes. As an experiment, I am leaving up the notes from the 2008 version of the course so that you can look ahead if you want. I will update these as the course progresses. Be aware that I have rearranged the course material a bit this year, so you may have to hunt for the appropriate lecture notes.

Any student who has a need for accommodation based on disability should contact me privately as soon as possible. Also contact the office of Disability Resources at 204-1280.

Biology 3096 - Cell Structure - Syllabus, Approximate.

A few definitions of symbols in the reading lists:

-> from this page until the next one on the list

ff "and pages following"

Date

Topic

Karp, 6th edition

August 29

Introduction to Cell Biology

1-30

31

Microscopes I (Resolution and EM)

728->

September 2

Microscopes II (Brightfield and Phase)

->

7

Fluorescence Microscopy

743

9

Principles of Molecular Organization: Bonds, Carbohydrates, Lipids, Nucleic Acids

http://www.chemguide.co.uk/atoms/bondingmenu.html#top

31-49

12

Water, Proteins, e.g. Antibodies ,

49-> Web site for molecular structure

14

More on Protein Structure,  

76

16

 - Self Assembly  

77-82

19

Separations :Electrophoresis,

Lab Manual, 749-751

21

Centrifugation, Chromatography

744->

23

Chromatography, Immunological Techniques, Affinity , Enzyme Characteristics I

749, 94->

25

Enzyme Characteristics II,

->

28

Kinetics

->107

30

Enzyme inhibition, Bio membranes - History

 

October 3

EXAM I

 

5

Biomembranes - Current Concepts

125->

7

Membrane Structure,

146

10

Extracellular Matrix,

239-258

12

Transport

147-> Web Site for Ion Channel

 Link to Neutrophil Movie

14

Resting Potential, Junctions ,

164

17

Surface Modifications, Junctions,  .

260-268, 311-325

19

Nuclear membrane, Endomembrane system, etc.,

486-494, 274-277

21

  Directed Assembly

281->

24

Post Translational Modifications

 

26

Dynamics- going out:  Sorting and Targeting

 

28

Dynamics – going in:  Phagocytosis, Pinocytosis, Autophagy

 

31

More on the Lysosome, protein turnover

 

November 2

Microtubules, Intermediate Filaments

->366

4

Muscle, Microfilaments

368->

7

EXAM II

 

9

Excitation-Contraction Coupling ,

384

11

Glycolysis, Metabolism, ^G

107-117

14

Citric Acid Cycle, Mitochondria, Oxidative Phosphorylation

183->

16

OxPhos, Photosynthesis

208

18

Photosynthesis

214-236

21

Cell to Cell Signals Ligand-Cell Surface Interactions I, Steroid Receptors

Figure 12.44, p525, 616->

23

Ligand-Cell Surface Interactions II

657

28

The Cell Cycle

307,

30

The Cell Cycle I

571->

December 2

The Cell Cycle II, Apoptosis

579, 653-657

5

Cancer I

662->687

7 (last day of classes)

Cancer II

 

The Final Examination will be December 14 (Wednesday) at 10:30 a.m.



LAB SCHEDULELABS BEGIN THE WEEK OF SEPTEMBER 5!

Week of:

Subject

September 5

Introduction, Microscopes I - Bright Field

September 12

Microscopes II - Phase and Darkfield

September 19

Protein Purification - Column

September 26

Electrophoresis - SDS-PAGE

October 3

Protein Purification Simulation

October 10

Cell Culture

October 17

Microscopes III - Fluorescence

October 26

Properties of Muscle Cells

October 31

Cell Fractionation I

November 7

Cell Fractionation II

November 14

Image Processing