Ph.D. Thesis on "Biochemical studies on sperm maturation in rhesus monkey", 1994. All India Institute of Medical Sciences, New Delhi, India. 

M.Sc. Biochemistry, First class, 1987, Post-Graduate Institute of Basic Medical Sciences, University of Madras, Madras, India. 

B.Sc. Zoology, Chemistry and Botany, First class, 1985, Madurai Kamaraj University, Madurai, India. 

C.G.T. Certificate in Gandhian Thought, 1984, Madurai Kamaraj University, Madurai, India. 

Assistant Professor,
Sol Sherry Thrombosis Research Center, Temple University, July 2005 - Present.

Associate Scientist,
Sol Sherry Thrombosis Research Center, Temple University, Dec. 2005 - Present.

Research Post-doctoral Fellowship,
Sol Sherry Thrombosis Research Center, Temple University, Nov. 2000 – June 2005. 

Research Post-doctoral Fellowship,
Fels Institute for Cancer Research and Molecular Biology, Temple University, Aug. 2000 – Oct. 2000. 

Post-doctoral Associateship, 
Department of Biochemistry, University of Minnesota, July 1993 – July 2000. 

Senior Research Fellowship, 
National Educational Test, University Grants Commission, New Delhi, India, Sep. 1990 – July 1993. 

Junior Research Fellowship, 
National Educational Test, University Grants Commission, New Delhi, India, Sep. 1988 – Aug. 1990. 

Junior Research Fellowship, 
Department of Science and Technology, New Delhi, Government of India, Oct. 1987 – Aug. 1988. 

Second Prize for Proficiency in Botany, APSA College (Madurai Kamaraj University), for the academic year 1983-84. 

First Prize for Proficiency in Tamil literature, APSA College (Madurai Kamaraj University), for the academic year 1983-84. 

First Prize for Proficiency in Botany, APSA College (Madurai Kamaraj University), for the academic year 1982-83. 

Molecular biology techniques: 
Macromolecule isolations: Genomic DNA and RNA isolation from various cell and tissue sources, plasmid preparation from bacterial cultures. 

Macromolecule modifications and cloning: cDNA preparation, polymerase chain reaction (PCR) techniques, plasmid T-vector preparation, PCR product cloning and bacterial transformations. 
Yeast recombinational clonning. 

Gene sequencing and detection methods: Enzymatic DNA sequencing, enzymatic radio-labeling of nucleic acids by end-labeling and random priming etc., for PCR and hybridization techniques. Northern, Southern blottings and hybridization to study T cell receptor variable region gene usage in peptide specific T cell lines and in peripheral blood leucocytes and acetylcholine receptor subunit gene expressions in human and animal cells and tissues. 

Cell culture techniques: 
Yeast cell culture for recombinant protein expression. 

Cell line development: Developing polyclonal human T cell lines against whole protein molecules and synthetic peptides. 

Cell proliferation assays: Microproliferation assays to map T cell epitopes on proteins as well as to study the MHC restriction. 

Site-directed mutagenesis, protein expression and purification: 
Mutant recombinant protein expression by site-directed mutagenesis and wild-type protein expression in bacterial and yeast expression systems. Protein isolation by Ion exchange, Size Exclusion and Affinity column chromatography techniques using FPLC. 

Electrophoresis and related methods: 
Protein Electrophoresis: Agarose and polyacrylamide gel electrophoresis, isoelectric focussing (IEF), non-equilibrium pH gradient electrophoresis (NEPHGE), protein titration curves, immuno electrophoresis. 

Protein transfer and detection methods: Western transfer and dot-blot, enzymatic and radio-lectin probing on blot membranes, autoradiogram and fluorogram techniques and chemiluminescence detection methods. 

Enzyme and protease inhibitor study: 
Enzyme kinetics - K_m, K_i, K_a and K_d determination. 

Solid-phase cellular assays:
Cellular lectin enzyme immunoassay (CLEIA), cellular enzyme linked immunosorbent assay (ELISA) and cellular radio-lectin receptor assay. 

Cyto/histological methods: 
Histochemical techniques, immunocytochemistry, radioactive and nonradioactive in situ hybridization and lectin fluorescence detection techniques. 

Immunization methods: 
Adjuvant preparations, subcutaneous and abdominal immunization of laboratory animals, bleeding and validation of antisera. 

Chemical and enzymatic methods of radiolabeling of proteins and glycoproteins: Radioiodination (¹²⁵I) and tritiation (³H) and subsequent immunoprecipitation, lectin affinity precipitation of glycoproteins and separation. 

Practical experience gained from training program: 
Received an extensive practical training in Scanning and Transmission Electron Microscopy. 

"Biomolecular Interaction Analysis". 'BIACORE 2000' instrument operation, experimental design and data analysis.  Biacore, Inc., Picataway, New Jersey, USA. Dec. 2001. 

"An Introduction to Computing in Biological Sciences". Molecular Biology Computing Center. University of Minnesota, St. Paul, USA. Feb. 1994. 

National training program on "Scanning and Transmission Electron microscopy. Regional EM Facility, All India Institute of Medical Sciences, New Delhi, India. Nov. 1989. 

Workshop on "Sperm Antigens". National Institute of Immunology, New Delhi, India. Feb. 1988. 

National seminar on Impact of Environment on Health. Society of Young Scientists, All India Institute of Medical Sciences, New Delhi, India. June 1988. 

Winter school program on "Lasers in chemical and biological sciences". Indian Institute of Technology, New Delhi, India. Dec. 1987. 

Navaneetham D, Lei Jin, Robert E. Babine, Sherin S. Abdel-Meguid and Peter N. Walsh. 2003.
Molecular Interactions between the Kunitz Protease Inhibitory Domain of Protease Nexin 2 and the Catalytic Subunit of Factor XIa Revealed by X-Ray Crystallography and Mutational Analysis. American Society of Hematology. Blood 102;127a

Navaneetham D., Penn A.S., Howard J.F., Jr. and Conti-Fine B.M. 1998. Thymus and blood TCR Vb usage in myasthenia gravis patients. International Myasthenia Gravis Workshop. Satellite Congress of International Congress of Immunology. Mysore, India. p. 11. 

Navaneetham, D., Raju, R., Diethelm, B., Hoppe, R., Horton, R.M. and Conti-Fine, B.M. 1997. TCR Vb usage by acetylcholine receptor specific CD4⁺ T cells in myasthenia gravis. IX International Conference on Myasthenia Gravis and Related Disorders. A New York Academy of Sciences Conference, Santa Monica, California, USA. p. 20. 

Navaneetham, D., Penn, A.S., Howard J.F. Jr. and Conti-Fine, B.M. 1996.  Expression of the a7 subunit of the nicotinic acetylcholine receptor in normal and myasthenic thymuses. Cellular and Molecular Biology 42: S-121. 

Navaneetham, D., and Rajalakshmi, M. 1992. Terminal glycoconjugate changes during epididymal maturation in rhesus monkey by cellular enzyme immunoassay. Biology of Reproduction  46: 150. 

Navaneetham, D., and Rajalakshmi, M. 1992.  Evaluation of peroxidase activity in seminal plasma and in ejaculated spermatozoa of human and rhesus monkey.  Presented at International Conference on Fertility Regulation, Institute for Research in Reproduction, Bombay, India.  p. 141. 

Navaneetham, D., Ramakrishnan, P.R., and Rajalakshmi, M. 1990.  An enzyme immunoassay technique for quantitation of sperm surface lectin receptors.  Presented at International Conference on Perspectives in Primate Reproductive Biology, Indian Institute of Science, Bangalore, India.  p. 59. 

Conti-Fine, B.M., Wang, Z.Y., Raju, R., Howard J.F. Jr., and Navaneetham, D. 2000. Anti-acetylcholine receptor CD4⁺ T cells in myasthenia gravis: epitope repertoire and T cell receptor gene usage. Chapter 8: 73-93. In Myasthenia Gravis: Disease mechanisms and immunointervention. Edited by Christadoss, P. Narosa Publishing House, New Delhi, India. 

Raju, R., Navaneetham, D., and Conti-Fine, B.M. 1999. Update to: Rapid method for the elution and analysis of PCR products separated on high resolution acrylamide gels. Chapter 42: 365-369. In Expression Genetics: Differential Display. Pardee, A.B., and McClelland, M. (Editors), BioTechniques Books, Eaton Publishing, Natick, MA, USA.  (ISBN 1-881299-31-7). 

Conti-Fine B.M., Navaneetham, D. Lei, S., and Maus, A.D.J. 2000. Neuronal nicotinic receptors in non-neuronal cells: new mediators of tobacco toxicity? European Journal of Pharmacology 393:279-294. Abstract online

Conti-Fine, B.M., Navaneetham, D., Karachunski P. I., Raju, R., Diethelm-Okita, B., Okita, D., Howard, J.F., Jr. and Wang, Z-Y. 1998. T-Cell recognition of the acetylcholine receptor in myasthenia gravis. Annals of New York Academy of Sciences 841: 283-308. 

Navaneetham, D. and Conti-Fine,B.M. 1998. Augmented PCR amplification by thermally activated DNA polymerase - AmpliTaq Gold, in the presence of densifying  agents.

Navaneetham D, Jin L, Pandey P, Strickler JE, Babine RE, Abdel-Meguid SS, Walsh PN. 2005. Structural and mutational analyses of the molecular interactions between the catalytic domain of factor XIa and the Kunitz protease inhibitor domain of protease nexin 2. Journal of  Biological Chemistry 280:36165-36175. Full article from publisher

Yun TH, Baglia FA, Myles T, Navaneetham D, Lopez JA, Walsh PN, Leung LL. 2003. Thrombin activation of factor XI on activated platelets requires the interaction of factor XI and platelet glycoprotein Ib alpha with thrombin anion-binding exosites I and II, respectively. Journal of  Biological Chemistry 278:48112-48119. Full article from publisher

Wang, Y., Pereira, E.F., Maus, A.D., Ostlie, N.S., Navaneetham, D., Lei, S., Albuquerque, E.X. and Conti-Fine, B.M. 2001. Human bronchial epithelial and endothelial cells express a7 nicotinic acetylcholine receptors. Molecular Pharmacology 60:1201-1209. Full article from publisher

Navaneetham, D., Penn, A.S., Howard, J.F., Jr. and Conti-Fine, B.M. 2001. Human thymuses express incomplete sets of muscle acetylcholine receptor subunits that seldom include the d subunit. Muscle & Nerve 24:203-210. Abstract online

Navaneetham, D., Penn, A.S., Howard, J.F., Jr. and Conti-Fine, B.M. 1998. TCR Vb usage in the thymus and blood of myasthenia gravis patients. Journal of Autoimmunity 11: 621-633. Abstract online

Maus, A.D.J., Pereira, E.F.R., Karachunski, P.I., Horton, R.M., Navaneetham, D., Macklin, K., Cortes, W.S., Albuquerque, E.X. and Conti-Fine, B.M. 1998. Human and rodent bronchial epithelial cells express functional nicotinic acetylcholine receptors. Molecular Pharmacology 54: 779-88. Full article from publisher

Raju, R., Navaneetham, D., Protti, M.P., Hoppe, R., Horton, R.M., Howard, J.F., Jr. and B.M. Conti-Fine. 1998. Autoimmune CD4⁺ T-Cell in Myasthenia Gravis patients have restricted but individual heterogeneity of their TCR Vb usage. Annals of New York Academy of Sciences 841: 324-328. 

Raju, R., Navaneetham, D., Kellermann, S-A., Morris, J.C., McCormick, D.J. and Conti-Fine, B.M. 1997. TCR Vb usage of TSH receptor specific CD4⁺ T cells in Graves' disease patients and healthy humans. Journal of Autoimmunity 10: 479-489. Abstract online

Navaneetham, D., Penn, A.,  Howard, J.F., Jr. and  Conti-Fine, B.M. 1997. Expression of the a7 subunit of the nicotinic acetylcholine receptor in normal and myasthenic human thymuses. Cellular and Molecular Biology 43: 433-442. Abstract online

Raju, R., Navaneetham, D., Protti, M.P., Horton, R.M., Hoppe, B.L., Howard, J.F., Jr. and Conti-Fine, B.M. 1997. TCR Vb usage by acetylcholine receptor specific CD4⁺ cells in Myasthenia Gravis. Journal of Autoimmunity 10: 203-217. Abstract online

Navaneetham, D., Sivashanmugam, P. and Rajalakshmi, M. 1996. Solid-phase cellular lectin receptor assay to study sperm surface lectin binding sites. Current Science 70: 986-990. 

Navaneetham, D., Sivashanmugam, P. and Rajalakshmi, M. 1996. Changes in the binding of lectins to epididymal, ejaculated and capacitated spermatozoa of rhesus monkey. Anatomical Record  245: 500-508. Abstract online

Raju, R., Navaneetham, D., Okita, D.K., Diethelm-Okita, B., McCormick, D. and Conti-Fine, B. M. 1995. Epitopes for human CD4⁺ T cells on diphtheria toxin: structural features of sequence segments forming epitopes recognized by most subjects. European Journal of Immunology 25: 3207-3214. Abstract online

Raju, R., Hoppe, B.L., Navaneetham, D. and Conti-Fine, B.M.  1995.  Rapid method for the elution and analysis of PCR products separated on high resolution acrylamide gels. BioTechniques 18: 33-35.